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Bacterial Cell Wall & Differential Staining
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Virtual Microbiology Classroom of Science Prof Online
Bacterial Cell Wall Lecture Materials
These
are the Bacterial Cell Wall & Differential Staining Lecture materials of the Virtual Microbiology Classroom. The resources below are used to supplement an actual
16-week college microbiology course.
Page last updated 2/2016
SPO VIRTUAL CLASSROOMS
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How to Prepare a Bacterial Smear for Gram Staining
How to Prepare a Bacterial Smear for Acid-fast
(Ziehl-Neelsen) Staining
How to Prepare a Bacterial Smear for Endospore Staining
How to Do a Gram Stain
How to Do an Acid-fast
Ziehl-Neelsen Stain
How to Do an Endospore Stain
Tutorial on Compound Light Microscope Parts & Operation
DIFFERENTIAL STAINING LABORATORY IMAGES
The images linked here directly relate to this lecture topic and are designed to help students review and study that lab material while outside of lab.
MORE BACTERIAL CELL WALL & STAINING
Links to useful articles, animations and videos to help students better understand this topic.
- How to Use a Compound Microscope video by the TCCMediaCrew that is very helpful in reviewing what you learned about the microscope in lab.
- The Gram Stain: An Animated Approach, Cavanaugh & Keen, North Carolina State University.
- Acid-fast Stain Animated Tutorial: Another good video of the staining procedure.
- Chiang, C. et al (2010) "Drug-resistant TB: Past, Present & Future" Respirology, Volume 15, Issue 3, pages 413–432.
- Prokaryotic Cell interactive diagram from Cells Alive website.
You have free access to a large collection of materials used in a college-level introductory microbiology course. The Virtual Microbiology Classroom provides a wide range of free educational resources including PowerPoint Lectures, Study Guides, Review Questions and Practice Test Questions.
VIDEOS OF DIFFERENTIAL STAINING PROCEDURES
LEARNING OBJECTIVES
These lecture learning objectives are a study guide based on the VMC Bacterial Cell Wall Lecture PowerPoint Show:
- List the steps of the Gram stain, and with each step, describe what happens to the cells being stained.List the steps of the Gram stain, and with each step, describe what happens to the cells being stained.
- List the steps of the acid-fast stain, and with each step, describe what happens to the cells being stained.
- What bacterial controls could be used in the Gram Stain? Why would these be appropriate controls?
- What bacterial controls would be used in acid-fast staining? Why would these be appropriate controls?
- Diagram and describe both Gram-positive and Gram-negative bacterial cell wall structure.
- Diagram the cell wall structure of acid-fast bacteria.
- What is betalactamase? What are betalactam antibiotics? How does betalactamase interfere with bacterial cell wall structure?
LECTURE POWERPOINT
Versions of Bacterial Cell Wall PPT:
- Student's Bacterial Cell Wall PowerPoint Show
- Student's Bacterial Cell Wall PowerPoint PDF Printout
Printing: The best way for students to print out the PowerPoint Show is to download the PDF version. Select Print, and, when the Print screen comes up, go to the Print Handling options. Under Page Scaling, select "Multiple pages per sheet" Under Pages Per Sheet, select "2". That uses up less paper, placing two slides on each page.
BACTERIAL CELL WALL HOMEWORK ASSIGNMENT
Word documents of a essay questions given to students at the end of the lecture. This tool helps the students review the main messages of the lecture while allowing the instructor to assess the class' understanding of the material.
- coming soon!
REQUIRED READINGS
Here is what you need to read, in addition to the PowerPoint, to master this topic. Check Moodle & your textbook for additional readings.
- Gram stain, Lab Notes from the Virtual Microbiology Classroom
- Acid-fast stain, Lab Notes from the Virtual Microbiology Classroom
- Differential Bacterial Stains: Gram, Acid-fast & Endospore
- Steinbach, WJ & Shetty AK(2001) "Use of the diagnostic bacteriology laboratory: a practical review for the clinician," Postgrad Med J 2001;77:148-156.
PRACTICE TEST & REVIEW QUESTIONS
These multiple-choice / matching practice test questions and essay review questions designed to help you better understand the material from this lecture.
The Bacterial Cell Wall Lecture materials include:
- a list of learning objectives
- a PowerPoint lecture
- a homework assignment
- "class note" articles
- practice test questions
Gram-positive Staphylococcus epidermidis @ 1000xTM+
Gram-negative Escherichia coli @ 1000xTM
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