Bacterial Cell Wall & Differential Staining
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Virtual Microbiology Classroom of Science Prof Online

ANAT & PHYSGENERAL BIOCELL BIOMICRO 8wkMICRO 15wkINSTRUCTORS

Bacterial Cell Wall Lecture Materials

These are the Bacterial Cell Wall & Differential Staining Lecture materials of the Virtual Microbiology Classroom. The resources below are used to supplement an actual 16-week college microbiology course. 

Microscope Slide with Gram Stained Bacterial Smears

Page last updated 2/2016
Instructor's Corner
SPO VIRTUAL CLASSROOMS
How to Prepare a Bacterial Smear for Gram Staining
How to Prepare a Bacterial Smear for Acid-fast 
(Ziehl-Neelsen) Staining
How to Prepare a Bacterial Smear for Endospore Staining
How to Do a Gram Stain
How to Do an Acid-fast 
Ziehl-Neelsen Stain

How to Do an Endospore Stain
Tutorial on Compound Light Microscope Parts & Operation




Photographic guides to differential stains 
now available!
1. Gram
2. Acid-fast
3. Endopsore

DIFFERENTIAL STAINING LABORATORY IMAGES
The images linked here directly relate to this lecture topic and are designed to help students review and study that lab material while outside of lab. 


MORE BACTERIAL CELL WALL & STAINING
Links to useful articles, animations and videos to help students better understand this topic.





Virtual Microbiology
Classroom






You have free access to a large collection of materials used in a college-level introductory microbiology course. The Virtual Microbiology Classroom provides a wide range of free educational resources including PowerPoint Lectures, Study Guides, Review Questions and Practice Test Questions.
Prokaryotic Cell, Mariana Ruiz
VIDEOS OF DIFFERENTIAL STAINING PROCEDURES
​LEARNING OBJECTIVES
These lecture learning objectives are a study guide based on the VMC Bacterial Cell Wall Lecture PowerPoint Show:

  • List the steps of the acid-fast stain, and with each step, describe what happens to the cells being stained. 
  • What bacterial controls could be used in the Gram Stain? Why would these be appropriate controls?
  • What bacterial controls would be used in acid-fast staining? Why would these be appropriate controls?
  • Diagram the cell wall structure of acid-fast bacteria.
  • What is betalactamase? What are betalactam antibiotics? How does betalactamase interfere with bacterial cell wall structure?


LECTURE POWERPOINT
Versions of Bacterial Cell Wall PPT: 

Printing: The best way for students to print out the PowerPoint Show is to download the PDF version. Select Print, and, when the Print screen comes up, go to the Print Handling options. Under Page Scaling, select "Multiple pages per sheet" Under Pages Per Sheet, select "2". That uses up less paper, placing two slides on each page.


BACTERIAL CELL WALL HOMEWORK ASSIGNMENT
Word documents of a essay questions given to students at the end of the lecture. This tool helps the students review the main messages of the lecture while allowing the instructor to assess the class' understanding of the material. 
  • coming soon!


REQUIRED READINGS
Here is what you need to read, in addition to the PowerPoint, to master this topic. Check Moodle & your textbook for additional readings.

  • Gram stain, Lab Notes from the Virtual Microbiology Classroom


PRACTICE TEST & REVIEW QUESTIONS
These multiple-choice / matching practice test questions and essay review questions designed to help you better understand the material from this lecture.

The Bacterial Cell Wall Lecture materials include:
  • a list of learning objectives
  • a PowerPoint lecture  
  • a homework assignment
  • "class note" articles 
  • practice test questions


Gram Positive Bacteria, Staph. epidermidis @1000xTM
Gram-positive Staphylococcus epidermidis @ 1000xTM+
Gram Negative Bacteria, E. coli @ 1000xTM
Gram-negative Escherichia coli @ 1000xTM
MICROBIOLOGY HOMEWORK ASSIGNMENTS

1. Fresh Air audio interview of Dr. Martin Blaser; Missing Microbes Homework Assignment

2. "Rodney vs Death" Radiolab episode; Homework Assignment

3. "Shrink" Radiolab episode; HW Assignment

4. "Patient Zero" Radiolab episode; HW Assignment

5. "Parasites" Radiolab episode, HW Assignment

6. Bacteria Classification Spreadsheet

7. Differential Media & Stains Study Table 
(Ex Cr)

8. "Contagion" Movie Assignment
(Ex Cr)

​9. CRISPR Radiolab episode, HW assignment


Monsters Inside Me TV Show on Parasitism from Animal Planet

Monsters Inside Me
TV show, from Animal Planet, presenting case studies of  people with parasitic infections. So interesting, you'll learn about parasitism without even trying!
Check out our FREE 
Monsters Inside Me 
Homework Assignments
 Part 1 & Part 2!